Isotopes are atoms of the same element that have different numbers of neutrons but the same number of protons and electrons. The difference in the number of neutrons between the various isotopes of an element means that the various isotopes have different masses.
In all living organisms, in substances like carbohydrate, starch, urea etc. To prove sugar contains carbon, take a little amount of sugar in spoon and heat it gently till it melts and turned into yellowish color. On further heating it changes into brown colored mass and then black, which is charcoal.