When one frog's heart is stimulated and the extract from this stimulated heart is transferred to the heart of another non-stimulated frog, the rate of heart beat of the second frog will be increased too because of the transmission of the impulses via the synapses. Hence, this observation proves that the transmission of the impulses at the synapses is a chemical event.
Raising temperature generally speeds up a reaction, and lowering temperature slows down a reaction. However, extreme high temperatures can cause an enzyme to lose its shape (denature) and stop working.
Each enzyme has an optimum pH range. Changing the pH outside of this range will slow enzyme activity. Extreme pH values can cause enzymes to denature.
Increasing substrate concentration also increases the rate of reaction to a certain point. Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.